[A fatal case of septicemia by serotype A:3 Pasteurella multocida in human: review of 14 septic cases in Japan].

We report a case of systemic inflammatory response syndrome (SIRS) due to septicemia by Pasteurella multocida (P. multocida) who died three days after on admission. A few cases of septicemia by P. multocida were previously reported. In particular, capsular serotype analysis and somatic serotype analysis of P. multocida in human is very rare. Serotype A:3 P. multocida was identified in a diabetic patient with chronic obstructive pulmonary disease (COPD). There was high possibility that the isolate has been transmitted from an indoor companion dog since the patient had had close contact with the dog for one month. Here we discuss our case and 13 human cases of septicemia by P. multocida reported in Japan. Four cases from dogs and eleven cases from cats were included in these cases. The mortality rate of septicemia by P. multocida was 21.4% (3/14 cases). Two out of three cases died were diabetic.

[Detection of monoclonality of gastric MALT lymphoma using PCR method and its clinicopathological application].

Twenty cases of H. pylori-related gastric lymphoproliferative disorders including 12 cases of MALT lymphoma (Grade 5 after Wotherspoon, et al), 6 of suspicious of MALT lymphoma (Grade 4), and 2 of active chronic gastritis (Grade 3) were studied. Using the nested PCR method, paraffin-embedded gastric biopsy specimens of these 20 cases were investigated whether or not monoclonal IgH rearrangement could be demonstrated in infiltrating lymphoid cells. Monoclonal IgH rearrangement was recognized in 6 of 12 MALT lymphomas, but in other 14 cases including 6 of MALT lymphomas the monoclonality was not recognized. The result showed the sensitivity was 50% and the specificity was 100% with this method. Follow-up study after eradication of H. pylori was performed on 6 cases of MALT lymphoma which had showed the monoclonality before, using the same method. The monoclonality disappeared in 5 of 6 and histology showed a complete remission. In the remaining one case the monoclonality was yet demonstrated and lymphoma cells were histologically recognized. Thus, this method is very useful to assess not only the histological diagnosis of MALT lymphoma but also the effectiveness after treatment.